Compare polymerases - (Jan/06/2009 )

How would you compare phusion (Neb), pfu (stratagene) and LA Taq (takara) polymerases?
I need to amplify a retroviral insert of unknown size (>4 kb) from genomic DNA (obtained from cultured cells), specificity more important than error rate.
Is there any difference between these 3 polymerases really? Has anyone experience with more than one of these?

Hi

I have used pfu and phu.

I generally use pfu for GC rich DNA and Phu for AT rich. I have had problems amplifying AT rich DNA with Pfu.

TC

We mostly use LA Taq of Takara for cloning in our lab. In my case, the longest one was just 3kb and it worked quite well with an average of about 3-4 point errors, but finally I had a perfect one