Restriction Mapping - Need help in clearing concept (Jan/02/2009 )
Could somebody make me understand in simple words what is actually restriction Mapping and what ared its application.
Please just try to make my concept clear about it.
Its simple maths. Just identify the restriction sites that cut your gene, find out the size of the fragments that would be released when you digest it (manually substract the numbers where teh enzyme cuts) and match with the experimental digests of yr gene with that particular enzymes. Do it with all the restriction sites and you have restriction mapped yr gene.