IEF without puting oil on IPG strip? - has anyone done that???:( (Dec/14/2008 )
Hi,
I made this stupid mistake with my important sample 
....actually I wanted to run a 24 cm gel and was doing it for the first time, as earlier i used to run 13 cm gels. Everything was set, added evrything except the dry strip cover fluid, switched on the IPGPhor instrument and dint realize it untill it reached 70000vhs.....now what?? Has anyone ever done that?????what shud i do ...shall i go for second dimension or not? I don't think I'll get anything but at least something 
........suggest me something:(
-amj2-
QUOTE (amj2 @ Dec 14 2008, 09:31 AM)
Hi,
I made this stupid mistake with my important sample ....actually I wanted to run a 24 cm gel and was doing it for the first time, as earlier i used to run 13 cm gels. Everything was set, added evrything except the dry strip cover fluid, switched on the IPGPhor instrument and dint realize it untill it reached 70000vhs.....now what?? Has anyone ever done that?????what shud i do ...shall i go for second dimension or not? I don't think I'll get anything but at least something ........suggest me something:(
I made this stupid mistake with my important sample
....actually I wanted to run a 24 cm gel and was doing it for the first time, as earlier i used to run 13 cm gels. Everything was set, added evrything except the dry strip cover fluid, switched on the IPGPhor instrument and dint realize it untill it reached 70000vhs.....now what?? Has anyone ever done that?????what shud i do ...shall i go for second dimension or not? I don't think I'll get anything but at least something ........suggest me something:(you know that you need excellent conditions to get a good performance; only, if the sample(s) which you sepatared are so unique that you cannot repeat 2D GE you should go on; otherwise, I would start again...
-The Bearer-