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Complete mitochondrial depolarization needed - (Dec/06/2008 )

Dear Forum users,
please could you advice me how to reach complete mitochondrial depolarization in living cells? I am trying antimycin and rotenon but they are shining red with JC1 as control.
Thank you.

-karpatov-

FCCP will do it, rotenone and antimycin only inhibits electron transport chain

QUOTE (karpatov @ Dec 7 2008, 02:08 AM)
Dear Forum users,
please could you advice me how to reach complete mitochondrial depolarization in living cells? I am trying antimycin and rotenon but they are shining red with JC1 as control.
Thank you.

-tertu-