Help: baculovirus expression system? Anyone's used it? - Has anyone used this system (Nov/02/2004 )

has anyone used Baculovirus expression system...share experience....

I have used the invitrogen Bac-to-Bac baculovirus expression system in Sf9 insect cells. From my experience, it worked great. The level of expression in the Sf9 cells is very high. Also, post translational modification in the cells is comparable to that of mammalian cells. I cultured the sf9 cells in suspension, however you can culture them adherently but it would be less convenient because you would need a lot of plates to get the number of cells you need.