BSA is able to stop an enzymatic reaction? - (Dec/02/2008 )

Does anyone knows how to explain me how a Buffer containing BSA is able to stop an enzymatic reaction?
The mixture then needs to be centrifuged?


Thanks in advance.

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BSA normally can't but the buffer possible can.
You have to give more specific information to answer the question.

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BSA or other albumins are used for several reasons in buffers and cell culture media; indeed, albumins are used to reduce protease activity against cell proteins; it is not really a stop but increasing the substrate for proteases, and therefore, protecting your proteins of interest;

also albumins may immobilize various lipophilic and hydrophylic moolecules (including proteins) that act as co-factors or effectors in enzymatic reactions

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