Miniprep mistake! can it be saved? - (Nov/28/2008 )
Hi,
I have just made a huge mistake with some very valuable DNA and eluted it from the miniprep column without performing the final wash step! (the one where you centrifuge to remove excess ethanol and wash solution). now my sample contains a large volume of ethanol and my sample and I was wondering if there was any way to remove the ethanol without harming the DNA?
Thank you in advance.
-Kami22-
QUOTE (Kami22 @ Nov 28 2008, 07:30 AM)
Hi,
I have just made a huge mistake with some very valuable DNA and eluted it from the miniprep column without performing the final wash step! (the one where you centrifuge to remove excess ethanol and wash solution). now my sample contains a large volume of ethanol and my sample and I was wondering if there was any way to remove the ethanol without harming the DNA?
Thank you in advance.
I have just made a huge mistake with some very valuable DNA and eluted it from the miniprep column without performing the final wash step! (the one where you centrifuge to remove excess ethanol and wash solution). now my sample contains a large volume of ethanol and my sample and I was wondering if there was any way to remove the ethanol without harming the DNA?
Thank you in advance.
U could go for one round of ethanol precipitation.
-Hanming86-
QUOTE
U could go for one round of ethanol precipitation.
but will this affect downstream applications?
-Kami22-
QUOTE (Kami22 @ Nov 28 2008, 07:43 AM)
QUOTE
U could go for one round of ethanol precipitation.
but will this affect downstream applications?
Assuming that you're good at EtoH precipitation it probably will enhance downstream applications because you get rid of the remaining salt contamination via a 70% etoh wash which is part of the EtoH precipitation .
-Hanming86-
QUOTE (Hanming86 @ Nov 28 2008, 04:59 PM)
QUOTE (Kami22 @ Nov 28 2008, 07:43 AM)
QUOTE
U could go for one round of ethanol precipitation.
but will this affect downstream applications?
Assuming that you're good at EtoH precipitation it probably will enhance downstream applications because you get rid of the remaining salt contamination via a 70% etoh wash which is part of the EtoH precipitation .
can i use isopropanol instead because I have quite a large volume to begin with?
-Kami22-
Hi, Well just centrifuge your DNA sample like ethanol precipitation and then remove the ethanol and wait for it to dry and add back in some water and do whatever you want with it.
-Travis-