promoter sequence - (Nov/21/2008 )

Hi,

How do I search for teh promoter sequence of my gene of interest. I know its genbank accession number and also its chromosome clone ID?

Thanks

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Blast your cDNA sequence against the genome (NCBI genome blast).

Sequence before your cDNA sequence is promoter sequence.

Work with 1-2 kb upstream of tss (transcription start site) in the beginning, but also look up 5-10-20 kb away for important motifs/binding sites.

hth/

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