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T4 Ligase problem - Please help me, (Nov/11/2008 )

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Try this, this would surely help.

Avoid agarose for yr vector, just digest, CIP the mix, column purify and use for ligation. The efficiency of ligation will be much higher. And screen more colonies, you will have religations, but a clone as well and hardly any junk colonies.


-T C-

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