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Curcumin - solubility of Curcumin in saline (Nov/10/2008 )

The problem we have is the solubility of Curcumin.
I am using bicarbonate-buffered as well as HEPES buffered solutions. However, at pH lower than 8.8 the solubility of Curcumin (final concentration 50┬Ámol/L; stock solution in DMSO 10mmol/L) is very limited. The stock solution once added to the bicarbonate-buffered Tyrode solution we are using for our experiments precipates (the added curcumin forms clouds within the solution right after adding it). The same happened in HEPES buffered solution, it still happened after using ascorbic acid as stabilizing agent and it only improved at highly alkaline pH values (~8.8).

Does anyone has experience with curcumin in Tyrode/saline solutions?

-curcuma longa-

QUOTE (curcuma longa @ Nov 11 2008, 01:26 AM)
The problem we have is the solubility of Curcumin.
I am using bicarbonate-buffered as well as HEPES buffered solutions. However, at pH lower than 8.8 the solubility of Curcumin (final concentration 50┬Ámol/L; stock solution in DMSO 10mmol/L) is very limited. The stock solution once added to the bicarbonate-buffered Tyrode solution we are using for our experiments precipates (the added curcumin forms clouds within the solution right after adding it). The same happened in HEPES buffered solution, it still happened after using ascorbic acid as stabilizing agent and it only improved at highly alkaline pH values (~8.8).

Does anyone has experience with curcumin in Tyrode/saline solutions?




If your assay will cope with higher amounts of DMSO that will help ( RMCD may also be an option). My favoured method is to use serum (BSA should also work) to hold curcumin in suspension (from 20mM stock in DMSO to 0.4mM in serum) but again it will depend on your particular assay limitations.

-Drt-