Basic Calculation Question - (Nov/06/2008 )

Hi there,

I am a little embaressed to ask this question but....

I am coating an ELISA plate and I want to coat at 100ng per well. Typically I make a 1ug/ml solution and load 100ul per well. My co-worker gave me 100ug of an antibody for coating and I am wondering the dilution to get it too 1ug/ml? In my mind the dilution factor is 100x, right? So for 2ml volume I would use 20ul?

Please let me know if this is correct!

Thanks.

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["I am coating an ELISA plate and I want to coat at 100ng per well. Typically I make a 1ug/ml solution and load 100ul per well. My co-worker gave me 100ug of an antibody for coating and I am wondering the dilution to get it too 1ug/ml? In my mind the dilution factor is 100x, right? So for 2ml volume I would use 20ul?"]

You are correct if you meant 100ug/mL. Otherwise you need to make up to an exact volumn.

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You have confused me!! Basically, I have 100ug of an antibody and I want to coat 2 wells of an ELISA plate with 100ng per well. How do I make the coating antibody solution?

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is your antibody in solution or dry?

if it is in solution, in what volume is it?

this will give you the starting concentration.

then you can use c1v1=c2v2 to determine the volume you require.

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