# Basic Calculation Question - (Nov/06/2008 )

Hi there,

I am a little embaressed to ask this question but....

I am coating an ELISA plate and I want to coat at 100ng per well. Typically I make a 1ug/ml solution and load 100ul per well. My co-worker gave me 100ug of an antibody for coating and I am wondering the dilution to get it too 1ug/ml? In my mind the dilution factor is 100x, right? So for 2ml volume I would use 20ul?

Please let me know if this is correct!

Thanks.

-Newbie74-

["I am coating an ELISA plate and I want to coat at 100ng per well. Typically I make a 1ug/ml solution and load 100ul per well. My co-worker gave me 100ug of an antibody for coating and I am wondering the dilution to get it too 1ug/ml? In my mind the dilution factor is 100x, right? So for 2ml volume I would use 20ul?"]

You are correct if you meant 100ug/mL. Otherwise you need to make up to an exact volumn.

-Drt-

You have confused me!! Basically, I have 100ug of an antibody and I want to coat 2 wells of an ELISA plate with 100ng per well. How do I make the coating antibody solution?

-Newbie74-

QUOTE (Newbie74 @ Nov 6 2008, 08:49 PM)
You have confused me!! Basically, I have 100ug of an antibody and I want to coat 2 wells of an ELISA plate with 100ng per well. How do I make the coating antibody solution?

is your antibody in solution or dry?

if it is in solution, in what volume is it?

this will give you the starting concentration.

then you can use c1v1=c2v2 to determine the volume you require.

-mdfenko-