Protocol Online logo
Top : Forum Archives: : Molecular Cloning

phrGFP-1 - searching for vector sequence - (Oct/31/2008 )

Hello

I have a big problem. I want to use a phrGFP-1 vector from Stratagene

http://www.stratagene.com/vectors/maps/pdf/phrGFP_1.pdf

but as You can see on the page the company show only a part of the vector sequence: MCS 1 and MCS 2. My problem is that i want to clone my DNA fragment into MCS 1 but the second cloning place is 34nt downstream from the kozak sequence so I need to know the part of the hrGFP coding sequence. Stratagene can giv me a entire vector sequence but only when i buy this vector and this is very stupid in my opinion.

So my question is that maby one of You used this vector and can give me a sequence? I will be very very gratefull because i cant use other vector.

Best

leblaszczyk

-leblaszczyk-

QUOTE (leblaszczyk @ Oct 31 2008, 05:55 AM)
Hello

I have a big problem. I want to use a phrGFP-1 vector from Stratagene

http://www.stratagene.com/vectors/maps/pdf/phrGFP_1.pdf

but as You can see on the page the company show only a part of the vector sequence: MCS 1 and MCS 2. My problem is that i want to clone my DNA fragment into MCS 1 but the second cloning place is 34nt downstream from the kozak sequence so I need to know the part of the hrGFP coding sequence. Stratagene can giv me a entire vector sequence but only when i buy this vector and this is very stupid in my opinion.

So my question is that maby one of You used this vector and can give me a sequence? I will be very very gratefull because i cant use other vector.

Best

leblaszczyk


Why don't you find the hrGFP sequence on GenBank: this is the accession number: EU048698. Remember to reverse complement this sequence before use ^^.

-bio_VN-

QUOTE (leblaszczyk @ Oct 31 2008, 10:55 PM)
Stratagene can giv me a entire vector sequence but only when i buy this vector and this is very stupid in my opinion.

That is the stupidest thing i've heard in a while. Are they trying to sell you the vector or not? Amazing.

-killerkoz17-

QUOTE (killerkoz17 @ Nov 10 2008, 11:10 PM)
QUOTE (leblaszczyk @ Oct 31 2008, 10:55 PM)
Stratagene can giv me a entire vector sequence but only when i buy this vector and this is very stupid in my opinion.

That is the stupidest thing i've heard in a while. Are they trying to sell you the vector or not? Amazing.



They can give me a vector sequence but ONLY when i buy the vector!!!

-leblaszczyk-

I don't have the full sequence, but I do have a restriction map for the entire vector if that would be any help.

-Jimmers-

QUOTE (Jimmers @ Nov 13 2008, 04:26 PM)
I don't have the full sequence, but I do have a restriction map for the entire vector if that would be any help.



I have the restriction map but thanks anyway:)

-leblaszczyk-

Someone who has it should sequence it and deposit the sequence in Genbank.

-phage434-