Determing the size of a cDNA library - (Oct/29/2008 )

I just obtained glycerol stock for a plant cDNA library (plasmids are in E Coli). The library is described as amplified, normalized and is full length cDNA with 1.8 x 10^9 cfu/ml. However, how do I determine how big the library is? ----I want to make sure when I innoculated for plasmid purification that I use enough gylcerol stock to innoculate with that will ensure I have the whole library.
thanks
Lisa

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I guess my answer is a bit too late, but anyway. To check the library size you normally titer the library. You serially dilute the glycerol stock and plate it on normal agar plates (with antibiotics or what have you). The number of colonies that grow on a plate times the dilution divided by the volume you plated is the size of the library/ml. So if I dilute my library 10^8 times and plate 100 ul of this dilution and 10 colonies grow on this plate, the size of the library is 10^10 cfu/ml.
I hope it helps.
Cheers,
Miha

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thanks

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