Negative reading on Shimadzu - (Oct/28/2008 )
Hey guys,
My friend is measuring the purity and conc. of DNA of a Shimadvcu uV spectrophometer. after performing the blank , we keep getting negative absorbance . THe DNA source is from purified PCR result. The DNA was analyzed on gel before and it showed bright band ie adequate amount of DNA . so the negative reading is quite fishy.
As i am a user of nanodrop i am not really sure how to explain this situation .
Could anyone tell us what's the underlying problem for this?
thanks
-Hanming86-
Be sure the cuvettes are clean.
Check that the reading of the cuvettes filled with the solvent are the same.
Check with a spectrum that your not measuring at a slope.
For dummy's, I've seen the problem, be sure that you that blank and sample are in the correct cuvettes
-Gerard-