Too many colonies - Too many colonies (Oct/22/2008 )
For the cloning of my pcr product using restriction digestion, I have got about 3 colonies on my control plate but about 70 on my test plate. But when I check 8 colonies from the test plate, all did not contain any plasmid at all!!!!!!!!! Any idea what is happening?
Problem with your antibiotic selective plate ? contamination ?