Protocol Online logo
Top : Forum Archives: : Protein Expression and Purification

sandwich Ni column - (Oct/20/2008 )

I wonder whether it's possible to use a His-tagged antibody and attach it to the column and in the second step, purify the corrosponding antigen through it's binding to the attached antibody? kind of sandwich as in sandwich ELISA?


you are proposing a different way of preparing an immunoaffinity matrix.

we crosslink to protein A.