how to choose Premag PRL-TK,PRL-SV40,PRL-CMV - (Oct/19/2008 )
how to choose Premag PRL-TK,PRL-SV40,PRL-CMV?
We typically recommend using the vector that gives moderate, but measurable Renilla luciferase activity. Usually the pRL-tk is favored because it uses a promoter that is usually less strong than SV40 and CMV. This will vary depending on the cell line and the transfection conditions. This article may proide additional information that may be of use: http://www.promega.com/cnotes/cn017/cn017_09.pdf.
If you have any additional questions, please do not hesitate to contact Promega Technical Services.