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Why use 200 units of ligase? - I am going cuckoo lol (Oct/10/2008 )

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Add the equilibration buffer and shake the bottle. Allow the phases to separate. Leave the aqueous layer. Do not bother remixing the two layers after that. Pipet phenol or phenol:chloroform from below the aqueous layer. Store at 4C to slow down oxidation.


yup!! thanks!!

-nanu nana-

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