introduction to PCR - explanation (Oct/17/2004 )

Hello .......

please , I know what is RCR , but I don't know how does it work , why we have to take care for temperature , G+C content and why the control of temperature in the presence of a primer is important????

all these questions make me confused>>>may anyone help me?

Here are some animated PCR tutorials

http://www.dnalc.org/shockwave/pcranwhole.html

http://allserv.rug.ac.be/~avierstr/principles/pcrani.html

http://www.bio.davidson.edu/courses/Immuno...ash/RT_PCR.html

http://www.csuchico.edu/~jbell/Biol207/animations/pcr.html

http://www.abpischools.org.uk/resources/po...pcr/pcranim.asp

thanks alot....they were useful