how can I stain a slide like this - (Sep/26/2008 )

This is a sporophyte slide of the Marchantia sp. I wonder what chemical substance is used to stain the slide like this. How to stain the foot part red?

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as per http://www.doctorfungus.org/thelabor/HISTOPAT.HTM


A number of histologic stains are available that are routinely used to visualize fungi in tissue sections. While some of these are special fungal stains, others are of more general use but still help to observe the tissue reactions and/or the infecting fungus. The reactions involved and comments of particular interest for some of these stains are summarized in the TABLE.

Gomori methenamine silver (GMS), Gridley's fungus (GF), and periodic acid-Schiff (PAS) are special for and very efficient to visualize the fungi. Among these three, GMS is more advantageous since it stains old and nonviable fungal elements more efficiently than the other two. Hematoxylin and eosin (H&E) stain, on the other hand, is very useful to visualize the host's response but is not a special fungal stain. It does not stain most of the fungi, except the Aspergillus spp. and the zygomycetes. Thus, a combination of GMS and H&E is usually employed to visualize both the tissue reaction and the infecting fungus.


Mucin stains, like Mayer's mucicarmine, Southgate's mucicarmine and Alcian blue, stain the mucopolysaccharide capsule of Cryptococcus neoformans.
However, they are not specific for this particular fungus. Blastomyces dermatitidis and Rhinosporidium seeberi may also be stained with the mucin stains.

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Marchantia is not a fungus, it is a liverwort (plant).

I supposethe red colour could be oil red, that is a pretty common stain used in plants as far as I can recall. or it may be a conjugated secondary/tertiary IHC stain, but I am not a plant histologist.

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The green is most likely fast green and the red may well be Safranin O. Lignin stains red. the following chart may be useful.

http://www.emsdiasum.com/microscopy/produc...ains_chart.aspx

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