Some strands in axons are stained with DAPI. - I don't know what they are. (Sep/15/2008 )
I used DAPI to stain the nuclei in my IHCs , the sections are from ganglia; some thing weird to me is that in addition to nuclei which are very nicely stained I can clearly see some strands in the axons; I suppose we shouldn't expect any DNA in axons. Does any body know what they are?
I'm also new to DAPI staining. but I know that DAPI binds to AT of DNA and AU of RNA.
sometimes when I stain my cells i can see that some parts of cytoplasm are also stained, this might be due to mitochondrial DNA or the RNA inside the cytoplasm.
also try to wash your samples more with PBS to get rid of unbound DAPI, although it only gives higher light emission when it binds to DNA/RNA.
Could you post those pic. here so we could see it. Thanks
I've been trying to post the picture a couple of times, it can't be uploaded although I'm not exceeding the size limit ( it's just about 10 kb) ...