thaw samples? - (Aug/31/2008 )
Hi! Just a general question how the best way is to thaw samples, on ice? warm by hand? For example serum, tissue of faeces samples, for RNA extraction or for antibodies. What do you think. Does it really matter?
I thaw serum at 4 deg.
Inappropriately thawed, U may lose antibodies as cryoprecipitates.
What about thaw on my hand or at room temperature of primers and plasmid samples stored at -20 deg? That's the way I use to do it, but I wonder if that is dangerous to my primers and plasmid.
DNA Samples and primers - I also thaw in hand. There was a thread where it was discussed some time ago.
Thawing serum Thread http://www.protocol-online.org/forums/inde...p;hl=thaw+serum
Thawing for PCR http://www.protocol-online.org/forums/inde...amp;hl=thaw+PCR
Interesting. I was told once that the best way to thaw serum samples was by heating by hand. Hear I read the opposite. The conclusion that it is not so important...?
But what samples for RNA extraction, always best to thaw on ice even if it takes a while?