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DH5alpha growth curve - Measuring absorbance at 620 nm (Aug/28/2008 )


I was wondering if someone could tell me how the absorbance at 620 nm for monitoring growth of DH5alpha compares to absorbance at 600 nm.



I guess it's a matter of what OD you can monitor on the device you have.
Since measuring OD600 (OD620) is measuring visible light scattering, I don't think there is much difference between both.


There won't be much difference between the two, both are in the orange/red range and strongly attenuated by particles in suspension.