Maxiprep problem - Add ethanol before isopropanol (Aug/15/2008 )
As described in title I added ethanol before isopropanol by mistake.
Now i am centrifuging the eluted DNA with isopropanol (10ml) and ethanol (5ml)
does it matter for the final DNA yeild?
Could you write down what has happened? I hope you don't mind as I can't quite imagine what you are doing.
You require more EtOH to precipitate a volume of DNA solution compared to Isopropanol. What are the volume ratio EtOH to DNA solution.
If you are using a mix of alcohol, just increase the volume of isopropanol to the volume that should have been used. More alcohol doesn't do anything but cost more money.