help! accidentally used the wrong secondary antibody - ICC (Jul/25/2008 )

Hi everyone,

I need help! I accidentally used a wrong secondary antibody on my double stainings today! Is there a change that restaining with the correct antibody will still work?

thx!

IMVHO it should work.

Might affect the result a little - depends on whether the secondary Ab U used binds the target. Make negative control accordingly so that U can compare if the result has been affected. Even if the target cells do not have the ligand for the secondary Ab, there can be non-specific binding. Try comparing with control.

Hi everyone,

I need help! I accidentally used a wrong secondary antibody on my double stainings today! Is there a change that restaining with the correct antibody will still work?

thx!

it depends on specficity of the wrong antibody u used and
which step was you when u switched to right antibody.....this both factor will affect ur experiment result

HI
If you use wrong it wont bind. unfortunatly if it binds it is impossible to change the bonding

njoy
s

Hi everyone,

I need help! I accidentally used a wrong secondary antibody on my double stainings today! Is there a change that restaining with the correct antibody will still work?

thx!