LPS floursecent studies - (Sep/23/2004 )

I would like to analyse the interactions of LPS with my cells, whether it is internalized etc.

Does anyone have a protocol?
Thank you.

Sigma sells FITC-conjugated LPS. You can stain your cells in the presence of this LPS-FITC, but beware, you must have serum present in your staining system, which will provide the LPS binding protein (LBP). Stain for 30 minutes on ice for cell surface and 30-60 minutes at 37C for internalization. You may need to play with the LPS concentration and timing.