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1. Mutagenesis - To amplify fragments for mutagenesis (reply: 1)
2. ABI real time PCR instrument fail rate - Wondering whether we need the (expensive) service contract (reply: 5)
3. PCR mixture - (reply: 2)
4. Primer desing for transcript variant - (reply: 2)
5. Contamination Problems! - (reply: 4)
6. PRC reagents and equipments - Is a PCR machine robust? (reply: 2)
7. SNP - How to design the SNP primer (reply: 1)
8. RT-PCR reagents KIT 1 or 2-step? - (reply: 3)
9. cost of DNA sequencer? - (reply: 2)
10. high temperature - high temperature (reply: 1)
11. microplate centrifuge - do we really need it and which one? (reply: 8)
12. Problems with making mix of PCR reagents ? - (reply: 4)
13. PCR - 16S Gene (reply: 3)
14. Help for Real-time PCR equipment choice - (reply: 4)
15. Corbett research palm cycler - (reply: 1)
16. iCycler-user on Rotor-Gene 3000 - how to run a temperature gradient? (reply: 1)
17. PCR machine choice: - (reply: 14)
18. pcr mutagenesis - (reply: 4)
19. PCR of pBluescript unknown insert - (reply: 1)
20. Primer Dilution. ...water or buffer? - (reply: 15)
21. PCR thermocycler (Eppendorf) - noise problem (reply: 1)
22. problem with optimized protocol - (reply: 3)
23. SCAR-PCR - (reply: 1)
24. GC rich oligo - (reply: 2)
25. ways to get a good gene amplification - (reply: 4)
26. cDNA and PCR - enzimes (reply: 1)
27. PCR optimization - (reply: 5)
28. Other than Taq? - for PCR after RT (reply: 3)
29. 96-well plates - (reply: 2)
30. Polymerase Choice Troubles - my primer melting temp is so low (~30 C) (reply: 4)
2. ABI real time PCR instrument fail rate - Wondering whether we need the (expensive) service contract (reply: 5)
3. PCR mixture - (reply: 2)
4. Primer desing for transcript variant - (reply: 2)
5. Contamination Problems! - (reply: 4)
6. PRC reagents and equipments - Is a PCR machine robust? (reply: 2)
7. SNP - How to design the SNP primer (reply: 1)
8. RT-PCR reagents KIT 1 or 2-step? - (reply: 3)
9. cost of DNA sequencer? - (reply: 2)
10. high temperature - high temperature (reply: 1)
11. microplate centrifuge - do we really need it and which one? (reply: 8)
12. Problems with making mix of PCR reagents ? - (reply: 4)
13. PCR - 16S Gene (reply: 3)
14. Help for Real-time PCR equipment choice - (reply: 4)
15. Corbett research palm cycler - (reply: 1)
16. iCycler-user on Rotor-Gene 3000 - how to run a temperature gradient? (reply: 1)
17. PCR machine choice: - (reply: 14)
18. pcr mutagenesis - (reply: 4)
19. PCR of pBluescript unknown insert - (reply: 1)
20. Primer Dilution. ...water or buffer? - (reply: 15)
21. PCR thermocycler (Eppendorf) - noise problem (reply: 1)
22. problem with optimized protocol - (reply: 3)
23. SCAR-PCR - (reply: 1)
24. GC rich oligo - (reply: 2)
25. ways to get a good gene amplification - (reply: 4)
26. cDNA and PCR - enzimes (reply: 1)
27. PCR optimization - (reply: 5)
28. Other than Taq? - for PCR after RT (reply: 3)
29. 96-well plates - (reply: 2)
30. Polymerase Choice Troubles - my primer melting temp is so low (~30 C) (reply: 4)