HEK293 for Adenovirus propagation - 293T, QB1 what's the difference? (Jul/11/2008 )
Hello fellows,
I've seen that people use HEK293T or HEK293 QB1 to propagate adenoviral vectors.
are those cell lines the same or is there any difference between them?
Thanks!!
-Marvilla-
For the purposes of adenoviral propagation, they are the same. QB1 cells are just a slight variant from some QBiogene, and the 293T cells contain part of SV40 T-antigen, that allows plasmids containing an SV40 promoter to be efficiently replicated.
-bob1-