i'm trying to transform competent dh5a by electroporation. but i'm having problems.the colonies that i pick and inoculate, are turning green?! has this happened to anyone?thanks

I ve had the same problem lately with DH5a. After transforming and inocculating the LB turns to green...somebody from invitrogen told me " We normaly recognize a green color because ofaccumulation of oxidized comlexes like FAD during Pichia Pastoris fermentation". This doesn't mean much to me but maybe it'sof any help to you? In my mind it's a contamination of some sort...

It can be a contamination with Pseudomonas, which is pretty common...

I ever met this problem. I inoculated the green colony to LB broth/amp100, It could not grow. but other DH5a grew well.

green colonies? Hmm that's very peculiar. Yea.. it can be contamination. Green colonies?