Mitochondrial isolation and storage. - How do I store (Jun/20/2008 )

Im isolating, for NMR, mitochondria from cells which I need to keep intact. Im using a Pierce Kit. I need to know two things. FIrstly, what kind of return does one normally get, ie 10 % or 80%?

The second thing is how is best to store them and when should they be used...within hours, days on ice etc etc.

Im sure someone here is more expert than I am.

Regards,
The Lost Irish scientist

Im isolating, for NMR, mitochondria from cells which I need to keep intact. Im using a Pierce Kit. I need to know two things. FIrstly, what kind of return does one normally get, ie 10 % or 80%?

The second thing is how is best to store them and when should they be used...within hours, days on ice etc etc.

Im sure someone here is more expert than I am.

Regards,
The Lost Irish scientist

QUOTE (johncarrigan @ Jun 20 2008, 02:53 AM)

Im isolating, for NMR, mitochondria from cells which I need to keep intact. Im using a Pierce Kit. I need to know two things. FIrstly, what kind of return does one normally get, ie 10 % or 80%?

The second thing is how is best to store them and when should they be used...within hours, days on ice etc etc.

Im sure someone here is more expert than I am.

Regards,
The Lost Irish scientist

See if you find such answers in these protocols:
http://search.vadlo.com/b/q?sn=158621799&a...ation&rel=0
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