Stripping of dry membrane? - can we do that? (Jun/03/2008 )
hi,
I have a Pierce stripping buffer and I want to reuse my membrane after visualization with NBT/BCIP and AP buffer.
the membrane is dry, I dried it two days ago, can I still use it for stripping? or the membrane should be wet to strip?
-Curtis-
I would wash it about 5min in PBS (or any simple unblocking washbuffer) before stripping, I guess you can't do anything wrong with that.
-jazim-
QUOTE (Curtis @ Jun 3 2008, 10:35 PM)
hi,
I have a Pierce stripping buffer and I want to reuse my membrane after visualization with NBT/BCIP and AP buffer.
the membrane is dry, I dried it two days ago, can I still use it for stripping? or the membrane should be wet to strip?
I have a Pierce stripping buffer and I want to reuse my membrane after visualization with NBT/BCIP and AP buffer.
the membrane is dry, I dried it two days ago, can I still use it for stripping? or the membrane should be wet to strip?
Very unlikely to work, unless protein of your interest lies in an area where antibodies have not bound during previous western
See results no. 3 and 4.
http://search.vadlo.com/b/q?keys=dry+membr...mp;sn=158621799
..
-cellcounter-