How to induce self-ligation of a vector? Self-Circularization? - (Jun/03/2008 )
Try to transform bacteria whith your small account of religated plasmid and innoculate it for making midi or miniprep
^^ Without an ori, transforming won't do anything.
Interesting project!! I don't really understand how it works so can't offer much help to get it done, but maybe we can figure out ways to minimize DNA loss? If I understood correctly, your biggest loss comes after ligation, where only 5% circularized? How do you dialyze? Can you ethanol ppt instead of speed vac'ing?
--> Wow, thanks for your answer. Sounds promising and I´ll try to religate by this method.