Which Kit should I use for microRNA microarray? - microRNA Microarray (May/14/2008 )
Hi All
I am a new one involve in MicroRNA.
I want to do a embryo tissue microRNA microarray.
Any suggestion about which Kit I should use for microRNA microarray?
Some body mentioned Abicom. Recently, invitrogen and sigma claimed that their kits are better. Any suggention about that?
Thanks in advance.
zeno
I am a new one involve in MicroRNA.
I want to do a embryo tissue microRNA microarray.
Any suggestion about which Kit I should use for microRNA microarray?
Some body mentioned Abicom. Recently, invitrogen and sigma claimed that their kits are better. Any suggention about that?
Thanks in advance.
zeno
We used the arrays from CombiMatrix with good results. Not sure about the others, but we'll be trying the Exiqon arrays soon.
I am sorry for my question is not clear.
My question is.
Which kit could I use to purify MiRNA from tissue for microRNA microarray?
First, I need to got miRNA. There are lots of kits. Ambicon, invitrogen. Sigma (they said they developed a wonderful kit for isolate MIRNA).
So, any suggestion?????????thanks
I've always used the Ambion mirVana kit with good results. For microarray samples I follow the total RNA isolation protocol and then enrich for small RNAs using the Fractionator from Ambion (it is expensive though :-( ).