TUNEL help - (Aug/27/2004 )
does anybody know whether or not you need to do a counterstain when you're performing TUNEL????? i seem to remember from years ago that you do but the promega protocol doesn't mention it??
I would suggest a light counterstain using either hematoxylin or methyl green. Please check protocols below:
Gill's Hematoxylin Solution
Methyl Green Solution
Note LIGHT counterstain. If over do it, positive cells well be covered.
I think you need to do a counter stain. You could refer to the manual given by roche-applied for their tunel kits.
All the best