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Mung Bean Nuclease - (Aug/26/2004 )


Who from you used Mung-Bean-Nuclease for polishing Restriction-Sites (In my case its a 3'overhang) blink.gif

What are/were your experiences? good/bad??

In our institute nobody seems ever to have used it.. unsure.gif unsure.gif



yeah, mung bean nuclease works great most of the time, just keep to the manufacturers datasheet.

Only once I had no - read: absolutely no- DNA left after I forgot a MBN digest for over half a day...