When dot blotting - (Apr/09/2008 )
Does the nitrocellulose need to be pre-wetted (like in traditional western blot), or can you just spot the proteins directly onto the dry nitrocellulose?
If I am testing antibody dilutions for westerns, I dot cell lysates onto dry membrane and then wet the membrane before blocking. It works
it will not really soak in; better you use a dot blot apparatus where you need no pre-wetting