Blots with glycopeptides - (Apr/07/2008 )

Hello there I have a question regarding Western or dot blot that I hope some one has some input about:

I need to detect whether animals immunized with glycopeptides (e.g. Vancomycin) have developed antibodies against these compounds - and I would like to use a blot for this. Does anyone have experience with doing blots with these types of compounds? Will they bind to the membrane themselves or should I link them to another compound?

And a final thing: What about smaller molecules like ampicillin? Can I get them to bind to the membrane by coupling them to a protein. (And which one would be appropriate?).

Thanks a lot.

Best regards,

Hello there I have a question regarding Western or dot blot that I hope some one has some input about:

I need to detect whether animals immunized with glycopeptides (e.g. Vancomycin) have developed antibodies against these compounds - and I would like to use a blot for this. Does anyone have experience with doing blots with these types of compounds? Will they bind to the membrane themselves or should I link them to another compound?

And a final thing: What about smaller molecules like ampicillin? Can I get them to bind to the membrane by coupling them to a protein. (And which one would be appropriate?).

Thanks a lot.

Best regards,

QUOTE (LLL @ Apr 7 2008, 07:27 AM)

Hello there I have a question regarding Western or dot blot that I hope some one has some input about:

I need to detect whether animals immunized with glycopeptides (e.g. Vancomycin) have developed antibodies against these compounds - and I would like to use a blot for this. Does anyone have experience with doing blots with these types of compounds? Will they bind to the membrane themselves or should I link them to another compound?

And a final thing: What about smaller molecules like ampicillin? Can I get them to bind to the membrane by coupling them to a protein. (And which one would be appropriate?).

Thanks a lot.

Best regards,

as I understand you have immunized more than one animal; for a first screening, do a dot (immuno)blot; may be you have a dot blot apparatus

proteins to couple peptides to immunize are low immunogenic such as albumine or KLH (keyhole limpit haemocyanin)