could anybody help me? - (Apr/04/2008 )
Can anyone tell me which vectors i can use to express proteins in S. cerevisia. I need a vector with a weak promoter and low copy number. I also want one vector with a strong promoter and high copy number to express my protein constitutively. It is OK if the vector has an inducible promoter. I searched these vectors online for long time, but i still have not found the ideal vectors.
Any suggestions will be greatly appreciated.
Thanks in advance!
Could anybody give me any suggestions? I really need to find these vectors within few days! Pleeeeezzzzzzzzzzzz!
There aren't many people online at the moment - you'll just have to be a bit patient
Finally, i found the vectors I need from ATCC catalogue online. I still write the way the found the vectors just in case that some others need to find out vector to use. It is really a good way to look for vector from ATCC catalogue online. They have the vectors for different species and for different markers.