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expression problem - expression (Mar/31/2008 )

hi all
can any body explain how to purify protien from the inclussion bodies in soluble form (active)
i expressed my protien in BL21 (DC3)in pET28a+ vector. but it goes to inclussion bodies and i need it in soluble form


See the QIAexpressionist (attachment). Some suggestions for how to solve this. Especially p. 53.