Please help me design BSP primer. - (Mar/23/2008 )
my bad tony, I thought I had attached the file.
here it is
Dear nick, thanks for your favor! I will try them!
Dear nick, reverse primers you suggested should be CCTTCTCCATAAAAAAAAA & ACAACTTAACGTATAAAAACAAAAAA. How can they act as reverse primers considering extensive A stretchs located in 3' end of both primers and serious dimer & crossdimer & and false priming?
that is the nature of bisulfite PCR and sequencing, I can understand your concern, by primer dimer does ot occur if you have a high Tm, they become irrelavent.
Dear nick, Thank you a lot! I will try it!