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Fluorescent microscopy - question about filter sets (Mar/20/2008 )

Dear all,
I would like to know the meaning of some information that is included in the filters for fluorescent scopes> for example, for DAPI, the excitation filter wavelenght is EX 360/40... what is the meaning of the "/40" pice of information? is that a +-40?

Thanks for your help guys!!
thegradstudent.

-thegradstudent-

Hi,
It means that it is 360+-20. So it is between 340 and 380.
Hope it helps,
Sayeh



QUOTE (thegradstudent @ Mar 20 2008, 02:45 PM)
Dear all,
I would like to know the meaning of some information that is included in the filters for fluorescent scopes> for example, for DAPI, the excitation filter wavelenght is EX 360/40... what is the meaning of the "/40" pice of information? is that a +-40?

Thanks for your help guys!!
thegradstudent.

-sayeh-

It's the entire bandwith, meaning that you go up and down by the half of 40 starting from 360. So a 510/10 bandpass filter would go from 505-515.

-vairus-

You guys are awesome! Thanks!

-thegradstudent-