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Freezing splenocytes - (Mar/13/2008 )

Hi guys,

I'm isolating splenocytes from mice. My problem is that I cant do all the experiments I'm planing to do on the same day and I might need to freeze my cells for further functional experiments (like cytotoxicity and ELISpot). Is it possible to freeze them? and if yes how and what media? or should I culture them but for how long?

Let me know please

-anwar_mt-

Hi,
I am not very sure but may be you can freeze them in the regular way in 90% FBS and 10% DMSO

-leelaram-

QUOTE (anwar_mt @ Mar 14 2008, 01:04 AM)
Hi guys,

I'm isolating splenocytes from mice. My problem is that I cant do all the experiments I'm planing to do on the same day and I might need to freeze my cells for further functional experiments (like cytotoxicity and ELISpot). Is it possible to freeze them? and if yes how and what media? or should I culture them but for how long?

Let me know please


Which cells do you want to use?

-Clare-

I'm not isolating specific cells. I will use all cells

-anwar_mt-