Protocol Online logo
Top : Forum Archives: : General Lab Techniques

Why we need to wash yeast with water when harvesting? - (Feb/13/2008 )

Hi, Everyone,

Does anybody know Why we need to wash yeast cells with water when harvesting them, but we don't need to wash bacteria when we harvest ? I need purify proteins from yeast cells. I grow them in YPD broth and I harvest them when OD 600 gets to 2.

Any replies will be appreciated very much!



Well, it's just to remove the YPD medium components. I suppose they can influence downstream applications.


Because yeast culture medium can become very acidic. I guess.