Why we need to wash yeast with water when harvesting? - (Feb/13/2008 )
Does anybody know Why we need to wash yeast cells with water when harvesting them, but we don't need to wash bacteria when we harvest ? I need purify proteins from yeast cells. I grow them in YPD broth and I harvest them when OD 600 gets to 2.
Any replies will be appreciated very much!
Well, it's just to remove the YPD medium components. I suppose they can influence downstream applications.
Because yeast culture medium can become very acidic. I guess.