o-NPG assay - (Feb/13/2008 )
Has anybody done o-NPG assay out there? I am trying to calculate the activity with the following formula:
[E420*total vol.* n (dilution)]/(4.5*vol. used for assay*time* protein content)
what i don't understand is where did the 4.5 come from?
-bigsky-
most likely the extinction coefficient.
-mdfenko-
QUOTE (mdfenko @ Feb 13 2008, 02:33 PM)
most likely the extinction coefficient.
thanks mdfenko
-bigsky-