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background staining in IHC ofartificial oralmucosa - (Jul/30/2004 )

I was trying to detect laminin 5 antigen in praffin embedded sections of artificially grown oral mucosa tissue(keratinocytes grown on fibroblasts enriched collagen 1 biomatrix) using DAKO envision kit.
i tried dilutions of polyclonal antilaminin 5 from 1:100 to 1:1000 still there is background staining.
can you suggest me how to overcome this?


Check this link:

IHC Troubleshooting


Thanks. I got good results afterwards.