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Concentrating Supernatant - (Feb/06/2008 )

Hi there,

a colleague is going to assay the contents of cell culture supernatant for native proteins by western blot... does anyone know of a kit or protocol to concentrate the proteins in a solution like this without interfering with their binding state?

many thanks


How about freeze-drying? Or centrifugal evaporation.


How about centricons? You can concentrate the sample, but you need to know the MW of your proteins to decide the cutoff of centricons