Question of QIAGEN's QIAquick Gel Extraction Kit - (Feb/04/2008 )
QUOTE (subtelo @ Feb 8 2008, 09:11 AM)
QUOTE (scolix @ Feb 7 2008, 04:46 PM)
QUOTE (perneseblue @ Feb 5 2008, 08:55 AM)
QUOTE (scolix @ Feb 4 2008, 10:17 PM)
Try adding 10ul of 3M sodium acetate just before adding isopropanol. We always added it while using Qiagen kit.
Hmm...interesting. Does that make a difference?
I have never actually compared them side by side. we never used Qiagen gel extraction kit without adding sodium acetate. My previous boss insisted on this step to everyone in the lab.
I did the comparision. Once the solution is yellow after the gel has been dissolved, there is no difference no matter of the addition of sodium acetate.
so you didnt notice any difference in DNA yield upon elution??? Its interesting !!!!