Tris tricine SDS page blotting - (Feb/01/2008 )

Hi,
I have run a tricine (10%) SDS gel and I would like to transfer it into nitrocellulose or PVDF membrane. Do I have to do the transfer immediately or can the gel be stored and transfered later? Can someone help me with that?
Thanks
Beluga

-beluga-

it is always better to transfer immediately than to take the chance that your proteins will diffuse while in storage (although there may be some who disagree).

-mdfenko-

QUOTE (mdfenko @ Feb 1 2008, 07:08 PM)

it is always better to transfer immediately than to take the chance that your proteins will diffuse while in storage (although there may be some who disagree).

Thanks for the answer. Can I transfer it using a wet system and with what kind of transfer buffer? Or do I have to do it using a semy-dry blotter?
Thanks

-beluga-

you can use whatever blotting system with which you are comfortable.

semi-dry will be faster and it is good for low mw proteins but wet blotting works at least as well, if a little slower.

-mdfenko-

QUOTE (mdfenko @ Feb 1 2008, 07:41 PM)

you can use whatever blotting system with which you are comfortable.

semi-dry will be faster and it is good for low mw proteins but wet blotting works at least as well, if a little slower.

Thanks a lot for your help!

-beluga-