Reducing temperature-dependence of probe? - (Jan/31/2008 )

The probe that we are currently using is very temperature-dependent. In fact, we can plot intensity vs. time and see curves similar to (but a reflection of) temperature vs time. Because of this, the temperature stability requirement is considerably high if smooth intensity curves are desired. Is there a manner to design probe to reduce its temperature-dependence? Probe design (molecular biology) is not my are of expertise, so any help would be much appreciated. Currently, purchase our probe from Gene Link.

Thank you all!

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What do you mean by 'probe' - pH? Expression?

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The probe we use is a TaqMan probe labeled with AlexaFluor 647 reporter dye and BHQ3 quencher dye. It is what allows us to monitor the fluorescence (indirectly monitoring amplicon). Is this what you are asking?

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