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Southern Blot - What is the purpose of the reagents?? (Jan/24/2008 )

Hello everyone,

I'm doing Southern blot and using different reagents/buffers. Could anyone tell me what is/are the purpose(s) of these buffers/reagents?

I've made SCC from NaCl and NaCitrate (by the way is there any difference b/w SCC and SSC or they are the same?).
Pre-hybridisation solution containing: SSC, Blocking reagent, N-Lauroylsarcosine and SDS
Buffer 1 having: Tris-HCl and NaCl
Buffer 2 having: buffer1 with blocking agent
Buffer 3 having: Tris-HCl, NaCl, MgCl2
Buffer 4 having: Tris-HCl and EDTA
and also we do washes with 2xSSC/0.1%SDS and then 0.2xSSC/0.1%SDS and 0.1xSSC/0.1%SDS

I really don't know what's been happening?

Thanks in advance


What do you think these buffers are for?

SSC and SCC are probably different, the only references I could find to SCC don't define what it is. I use SSC for all my southerns.

These questions look like homework to me, try the homework forum.